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Wagh, S. S.
- Effect of Different Culture Media, Temperature, Ph, Carbon and Nitrogen Sourceson Mycelial Growth and Sporulation of Alternaria Carthami Causing Alternaria Blight of Safflower
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Authors
Affiliations
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Prabhani (M.S.), IN
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Prabhani (M.S.), IN
Source
International Journal of Plant Protection, Vol 7, No 2 (2014), Pagination: 349-353Abstract
Eight culture media, eight temperature levels, nine pH levels, seven carbon and six nitrogen sources tested exhibited better growth of Alternaria carthami. Results revealed that Potato dextrose agar gave significantly highest growth (90.00 mm), followed by Potato malt agar (84.16 mm) with excellent sporulation. Temperature levels indicated that highest mean mycelial growth (85.66 mm) was recorded at 30°C followed by 25°C (83.83 mm) and 20°C (66.33 mm). However, maximum mean mycelial growth (85.83 mm) was recorded at pH 6.5 with excellent sporulation, followed by at pH 6 (82.00 mm) and pH 7 (70.33 mm) with excellent and good sporulation, respectively. The carbon sources exhibited varied radial mycelial growth and sporulation of the test pathogen. However, highest radial mycelial growth (86.00 mm) and excellent sporulation was recorded on glucose, followed by on maltose (82.83 mm) and starch (80.33 mm) with excellent sporulation. Nitrogen sources resulted highest radial mycelial growth (82.55 mm) and excellent sporulation on potassium nitrate, followed by on peptone (75.83 mm) with good sporulation. Least radial mycelial growth (19.00 mm) was recorded on urea with poor sporulation.Keywords
Alternaria Carthami, Mycelial Growth, Carbon And Nitrogen Sources- Effect of Fungicides, Botanicals and Bioagents against Purple Blotch of Onion Caused by Alternaria Porri
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Authors
Affiliations
1 Department of Plant Pathology, College of Agriculture, Latur (M. S.), IN
2 Department of Plant Pathology, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Prabhani (m.s.), IN
3 Department of Plant Pathology, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Prabhani (M.S.), IN
1 Department of Plant Pathology, College of Agriculture, Latur (M. S.), IN
2 Department of Plant Pathology, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Prabhani (m.s.), IN
3 Department of Plant Pathology, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Prabhani (M.S.), IN
Source
International Journal of Plant Protection, Vol 7, No 2 (2014), Pagination: 405-410Abstract
A study was conducted in the of Department Plant Pathology, College of Agriculture, Latur, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani, Maharashtra, India, during 2011 to control Alternaria porri causing Alternaria blight of onion with fungicides, botanical and bio-agents. Among nine treatments, six fungicides (@ 100, 200, 250 and 500 ppm concentrations), one plant extract and two bioagents (@ 500 ppm) were evaluated in vitro in vivo and were found effective against A. porri and recorded significant inhibition of the test pathogen over untreated control. However, in vitro result revealed that in hexaconozole cent per cent (100.00 %) inhibition was observed, followed by difenoconazole (83.91 %), mancozeb (63.58%), P. florescence (58.94 %) and T. viride (54.45%). The minimum per cent inhibition was observed in chlorothalonil (31.40 %) followed by plant extract NSKE (43.92 %), copper oxychloride (46.87 %) and carbandazim (47.11 %). In vivo results revealed that hexaconozole (0.1%) was found most effective and recorded significantly least mean disease incidence (6.03 %) and intensity (13.33 %) with corresponding significantly increased bulb yield (438.00 q/ha) followed by mancozeb (@ 0.2%) and copper oxychloride (0.25%) which recorded significantly mean disease incidence of 6.83 and 8.53 per cent and intensity, 15.00 and 20.00 per cent, respectively and gave correspondingly bulb yield, respectively of 375.00 and 429.00 q/ha. The botanical tested, A. indica (@ 5%) was found antifungal against A. porri and recorded significantly disease incidence (7.96 %) and intensity (27.00 %), and gave the bulb yield (290.00 q/ha). Both fungal and bacterial antagonists tested were found not so effective to reduce incidence and intensity, attempt increased the bulb yield over unsprayed control.Keywords
Onion, Alternaria Porri, Purple Blotch, In Vitro And in Vivo Evaluation- Growth, Development and Viability of Metarhizium Anisopliae on Media with Various Nutrient Sources
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Authors
Affiliations
1 Department of Entomology, Agricultural Research Station, Niphad, Nasik (M.S.), IN
2 Department of Entomology, Mahatma Phule Krishi Vidhyapeeth, Rahuri, Ahmednagar (M. S.), IN
1 Department of Entomology, Agricultural Research Station, Niphad, Nasik (M.S.), IN
2 Department of Entomology, Mahatma Phule Krishi Vidhyapeeth, Rahuri, Ahmednagar (M. S.), IN
Source
International Journal of Plant Protection, Vol 7, No 2 (2014), Pagination: 420-423Abstract
The entomopathogenic fungus, Metarhizium anisopliae (Metschinikoff) Sorokin was mass produced in different liquid media. The nine media of various nutrient sources were evaluated to find out the most suitable medium for growth, biomass and viability of M.anisopliae. Sabouraud's dextrose broth with yeast extract proved to be the superior which gave significantly highest cfu (12.33x108/ml) and biomass (7.20g). The next best medium was Sabouraud's maltose broth with yeast extract and potato dextrose broth with yeast extract which registering cfu count of (10.33 × 108 and 10.67 × 108cfu/ml) and biomass (6.27 and 5.73 g), respectively. The lowest (48.33%) medium surface coverage and least biomass (1.57g) and cfu (4.33 × 108/ ml) were registered in medium with malt extract. Thus, considering growth, development and viability of M.anisopliae, Sabouraud's dextrose broth with yeast extract (SDY) emerged as the most potential medium for biomass production.Keywords
Metarhizium Anisopliae, Yeast Extract, Biomass, Colony Forming Unit- In Vitro Evaluation of Fungicides and Botanicals against Stem Rot of Chilli Caused by Sclerotium Rolfsii
Abstract Views :267 |
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Authors
Amreen Begum
1,
M. S. Dadke
1,
S. S. Wagh
2,
D. P. Kuldhar
2,
D.V. Pawar
2,
A. A. Chavan
2,
D. S. Thaware
2
Affiliations
1 Department of Plant Pathology, College of Agriculture, Latur (M.S.), IN
2 Department of Plant Pathology, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M. S.), IN
1 Department of Plant Pathology, College of Agriculture, Latur (M.S.), IN
2 Department of Plant Pathology, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M. S.), IN
Source
International Journal of Plant Protection, Vol 7, No 2 (2014), Pagination: 437-440Abstract
Stem rot caused by Sclerotium rolfsii Sacc. has been observed to cause rapid mortality in chilli plantations. Among eight fungicides and eight botanicals tested in vitro against S. rolfsii, the result revealed that maximum (100%) inhibition was observed in carboxin, propiconazole, hexaconazole, difenconazole and carbendazim at all three concentrations viz., 500, 1000 and 1500 ppm followed by captan (79.30, 82.76 and 85.23%) and triadimenfon (49.13, 60.23 and 65.33%) over control. Minimum per cent of inhibition was observed in the plates poisoned with copper oxychloride (47.26, 51.63 and 54.40%), respectively at all three concentrations. Among botanicals, at 5 and 10 per cent concentrations, significantly highest average inhibition was recorded with neem (74.81%), followed by tulsi (67.10%) and nirgudi (65.81%). Significantly least average inhibition was recorded with sorghum (47.23 %). The rest of the botanicals recorded more than 50.00 per cent average inhibition of mycelial growth over untreated control (00.00%).Keywords
Chilli, S. Rolfsii, Stem Rot, in Vitro Evaluation, Fungicides, Botanicals- Effect of Different Culture Media, Temperature and PH on Growth and Sporulation of Alternaria carthami
Abstract Views :274 |
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Authors
Affiliations
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, PARBHANI (M.S.), IN
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, PARBHANI (M.S.), IN
Source
International Journal of Plant Sciences, Vol 10, No 2 (2015), Pagination: 163-167Abstract
All eight culture media tested encouraged better growth of Alternaria carthami. However, Potato dextrose agar gave significantly highest growth (90.00 mm). The second and third best culture media found were Potato malt agar (84.16 mm) and Yeast manitol agar (73.33 mm). Rest of the culture media recorded good amount of mycelial growth in the range of 41.66 mm (Yeast extract agar) to 69.16 mm (Malt extract agar). Colony growth was circular, cottony, grayishblack or olivaceous-black coloured with fair to excellent sporulation. The mean colony growth recorded with all the different temperature ranged from 5.66 mm at 5°C to 85.66 mm at 30°C. However, significantly highest mean mycelial growth (85.66 mm) was recorded at 30°C with excellent (++++) sporulation. The second and third best temperature found were 25°C (83.83 mm) and 20°C (66.33 mm). The mean colony growth recorded with all the pH values ranged from 30.50 mm at pH 4.0 to 85.83 mm at pH 6.5. However, significantly maximum mean mycelial growth (85.83 mm) was recorded at pH 6.5 with excellent (++++) sporulation. The second and third best pH values found were pH 6 (82.00 mm) and pH 7 (70.33 mm) with excellent and good sporulation, respectively.Keywords
Culture Media, Temperature, PH, A. carthami, Growth, Sporulation.- Nutritional and Physiological Studies of Fusarium oxysporum f. sp. Ciceri (Padwick) Snyder and Hansen Causing Wilt of Chickpea
Abstract Views :270 |
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Authors
Affiliations
1 Department of Plant Pathology, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN
1 Department of Plant Pathology, College of Agriculture, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN
Source
International Journal of Plant Sciences, Vol 11, No 2 (2016), Pagination: 213-217Abstract
Nutritional and physiological requirements of Fusarium oxysporum f. sp. ciceri were studied using most virulent isolate FOC-2 (Jalna). Effect of different culture media on mycelial growth of Fusarium oxysporum f. sp. ciceri showed significant differences in growth and sporulation. In vitro, culture media studied, Potato dextrose agar (89.66 mm) and Richard agar medium (85.66) produced significantly highest mean mycelial growth and sporulation. The temperature in the range of 25°C to 30°C and pH having range of 6.0 to 7.0 produce significantly highest mean mycelial growth and sporulation of the test pathogen.Keywords
Nutritional, Physiological, Chickpea.References
- Anonymous (2014). Directorate of Economics and Statistics, Department of Agriculture and Co-operation. Agricultural Statistics at a glance. pp. 94-96.
- Awachar, M.K. (2014). Studies on morphological variability of Fusarium oxysporum f. sp. ciceri causing wilt of chickpea. M.Sc. (Ag.) Thesis, MPKV, Rahuri, Ahmednagar, M.S. (INDIA).
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- Chavan, T.B. (2004). Studies on Fusarium oxysporum f. sp. ciceri (Padwick) Snyder and Hansen causing wilt of chickpea (Cicer arietinum L.). M.Sc. (Ag.) Thesis, Indira Gandhi Agriculture University, Raipur, C.G. (INDIA).
- Dhar, V. and Gurha, S.N. (1998). Integrated management of chickpea diseases. (Rajeev, K., Upadhyay, K. G., Mukerji, B. P., Chamola and Dubey, O. P. (edi.)), APH Pub. Co., New Delhi. (India). pp: 249.
- Gaikwad, S.J. and Pachpande, D.S. (1992). Effect of temperature on wilt of sesamum caused by Fusarium oxysporum f. sp. sesami. J. Man. Agril. Univ., 17(1): 76-78.
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- Jalali, B.L. and Chand, H. (1992). Diseases of cereals and pulses. (U. S. Singh, A. N. Mukhopadhayay, J. Kumar, and H. S. Chaube, edi.) Prentice Hall, Englewood Cliffs, NY. 1-429-444.
- Kadam, N. (2012). Molecular characterization of different isolates of Fusarium oxysporum f. sp. ciceri causing chickpea wilt from Maharashtra. M.Sc. (Ag.) Thesis, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, M.S. (INDIA).
- Khan, H.S., Saifulla M., Mahesh, S.B. and Pallavi, M.S. (2011). Effect of different media and environmental conditions on the growth of Fusarium oxysporum f. sp. ciceri causing Fusarium wilt of chickpea.
- Khilare, V.C. and Ahmed, R. (2012). Effect of different media, pH and temperature on the growth of Fusarium oxysporum f. sp. ciceri causing chickpea wilt. Internat. J. Advan. Bio. Res., 2(1) : 99-102.
- Khilare, V.C., Ahmed, R., Chavan, S.S. and Kohire, O.D. (2009). Management of Fusarium oxysporum f. sp. ciceri by different fungicides. Bioinfolet., 6 : 41-43.
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- Nene, Y.L. and Reddy, M.V. (1987). Chickpea diseases and their control. Phytopatholgy, 42: 499-505.
- Osman, M., Sayed, M.A., Mohamed, Y.A.H. and Metwally, M. (1992). Effect of various cultural conditions culture media, temperature and carbon source on Alternaria alternata and Fusarium oxysporum. Microbios, 71 (286) : 15-26.
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- Shadha, W.T., Rahma, A.A. and Rageh, S.A. (1995). Damping off of some cucurbitaceous crops in Saudi Arabia with reference to control methods. J. Phytopath., 6 (2): 125-129.
- Wereher, M. (1990). Effect of temperature and media composition on growth and sporulation of formae special of Fusarium oxysporum Schlecht. Recozniki Akademi Rolniczej-W. Poznaniu- Ogrodnictwo., 18: 107-125.
- Survey for Incidence and Severity of Alternaria Blight of Safflower in Different Districts of Marathwada Region
Abstract Views :145 |
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Authors
Affiliations
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN
Source
International Journal of Plant Protection, Vol 9, No 1 (2016), Pagination: 219-222Abstract
Survey was conducted in four districts of Marathwada region of Maharashtra during Rabi 2013 to know the incidence and intensity of leaf blight disease caused by Alternaria carthami. The disease was predominant in all the districts surveyed. Highest per cent disease incidence and intensity was reported in Hingoli (42.08 and 38.46 %, respectively). This was followed by Nanded (40.07 and 36.03 %, respectively).Keywords
Alternaria Blight, Incidence, Carthamus tinctorius, Severity Survey.- Isolation, Identification, Pathogenicity Test and Screening of Brinjal Cultivars against Damping off in Brinjal
Abstract Views :111 |
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Authors
Affiliations
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani (M.S.), IN
Source
International Journal of Plant Protection, Vol 9, No 1 (2016), Pagination: 246-251Abstract
Brinjal or egg plant (Solanum melongena L.) is widely grown vegetable crop in India. Of the various diseases affecting brinjal, damping off caused by Pythium ultimum Trow. is one of the most destructive diseases causing several yield losses. The pathogen (P.ultimum) from naturally diseased brinjal plant showing typical symptoms of damping off was successfully isolated on the basal culture medium potato dextrose agar. The fungus P.ultimum produce nonseptate, well branched, colourless to whitish mycelium, sporangia on indeterminate sporangiophores when observed under the microscope. Pathogenicity of Pythium ultimum Trow. was proved by sick soil method in pot culture, sowing brinjal cv. HADGAON LOCAL under screen house condition. The pathogen was reisolated on PDA from artificially diseased brinjal seedling, and compared its cultural and morphological characteristics with the original fungus isolated from the naturally damping off diseased brinjal plant. Based on the typical symptoms of damping off, morphological and cultural characteristics, microscopic observations and pathogenicity test; the test pathogen was identified and confirmed as P. ultimum. Results revealed that of 13 brinjal cultivar lines, five lines viz., EPM-564, Ajay, Kranti seed, Brinjal MG and Puneri Kateri were moderately susceptible with disease incidence in the range of 11.10 to 16.66 per cent and five lines viz., Vishal, Arnav, Local Pingali, Local Kinwat, Local Hadgaon were susceptible with the disease incidence of about 22.21 to 44.44 per cent.Keywords
Brinjal, Isolation, Pathogenicity, In vitro, Screening, Pythium ultimum.- Effect of INM on Nutrient Uptake, Yield and Quality of Okra [Abelmoschus esculents (L.) Moench]
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Authors
Affiliations
1 Department of Soil Science and Agricultural Chemistry, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), IN
2 National Bureau of Soil Survey and Land Using Planning, Nagpur (M.S.), IN
1 Department of Soil Science and Agricultural Chemistry, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola (M.S.), IN
2 National Bureau of Soil Survey and Land Using Planning, Nagpur (M.S.), IN